Gianluca Margheri graduated with honours in Musical Dramaturgy at Università degli Studi di Firenze, and in Vocal Chamber Music at the Cherubini conservatory in Florence. Over the years he has taken part in several master classes held by Carlo Miliciani, Josef Loibl, Sherman Lowe, Michele Pertusi and Ildebrando D'Arcangelo, Paolo Coni and Fernando Cordeiro.

After his stage debut in 2004 he has performed in many international opera houses - Teatro Regio in Parma, Teatro Filarmonico in Verona, Teatro del Maggio Musicale Fiorentino, Opera di Firenze, Teatro Real in Madrid, Hungarian State Opera, Teatro "Verdi" in Sassari, Teatro Comunale in Modena, Teatro "Valli" in Reggio Emilia, Teatro Sociale in Como, Teatro "Ponchielli" in Cremona, Arena di Verona, Opéra de Rouen, Opéra Saint-Étienne, Opera House in Curitiba, Opéra de Liège, Opera de Las Palmas, Bayerischer Rundfunk and "Festival Alte Musik" in Innsbruck, Teatro Massimo di Palermo, Teatro Lirico Cagliari and Rossini Opera Festival. In July 2009 he won the first prize at the international opera competition "Toti Dal Monte" in Treviso. His operatic repertoire mainly includes 18th and 19th century opera: from masterpieces by Monteverdi, Händel, Haydn, Rossini, Donizetti and Bellini, to the modern repertoire of Verdi, Puccini and Britten, all the way to contemporary Donatoni. Mozart's operas were highlights in his career, due to his interpretation of Guglielmo in Così Fan Tutte, the main character in Don Giovanni, Figaro and the Count of Almaviva in Le Nozze di Figaro. He has worked with conductors and directors James Conlon, Graham Vick, Zubin Mehta, Friedrich Haider, Jesús López-Cobos, Elio De Capitani, Riccardo Frizza, Daniele Rustioni, Andrea Battistoni, Roland Böer, Alan Curtis, Jonathan Webb, Lindsay Kemp, Gabriele Ferro, Giorgio Barberio Corsetti, PierLuigi Pizzi and many others. His discography includes Händel's Deidamia (CD, Virgin Classic) Stiava's Vespri di Santa Cecilia (CD, Tactus), Brahms' Ein Deutsches Requiem (DVD, DeAgostini), Pizzetti's Assassinio nella Cattedrale (DVD, Sony Music) and the Rossini Album "Amor fatale” next to Marina Rebeka with the Münchner Rundfunkorchester conducted by Marco Armiliato (BR Klassik)